Question 1

Work package WP1 – Project Management, dissemination, public engagement and exploitation

Accepted Answer

Objectives:
1) Supervision of the scientific progress towards the network's planned objectives;
2) Day-to-day financial and administrative management including secondments, reports, communication between partners and the EC;
3) Logistical support and follow-up of consortium meetings and related activities;
4) Communication and dissemination of project knowledge and results;
6) Supporting exploitation of results by partners towards their networks and beyond;
7)Promotion of gender balance in the consortium’s research teams.

Question 2

Work package WP2 – Training and secondment

Accepted Answer

Objectives:
1) Planning and coordination of network-wide training events;
2) Planning and coordination of local training events;
3) Planning and coordination of webinars;
4) Planning and coordination of individual and shared secondments.

Question 3

Work package WP3 – Acquisition and specification of extracellular vesicles (EVs) from biological sources

Accepted Answer

Objectives
1)Acquisition and proteomic analysis of EVs from patients with BC, LC and HC and from healthy donors;
2)Acquisition and proteomic analysis of EVs produced by normal or immortalized breast, and liver cell lines;
3) Acquisition and proteomic analysis of EVs produced by cell lines of BC, HC, and LC to select the cell lines that best fit pathological phenotype in patients;
4) Acquisition and analysis of plant EVs from various edible sources such as ginger, grape, lemon,
grapefruit etc to select the source with compromised in vitro immunogenicity

Question 4

Work package WP4 – Nanobodies (Nbs, recombinant variable domains of heavy-chain-only antibodies) against EVs: selection, optimization and production

Accepted Answer

Objectives:
Selection, and validation of the Nbs for EV purification unit

Question 5

Work package WP5 – Components and system integration for different scale

Accepted Answer

Objectives:
1) Development of novel EVs purification system based on NbS and solid carrier;
2) Validation of developed unit on different biological samples;
3) Optimising protocols for system application in large-scale EVs production
4) Optimising protocols for system application in high-throughput EVs isolation.

Question 6

Work package WP6 – Prototype validation in clinical laboratory settings

Accepted Answer

Objectives:
1) Validation of the PROTOTYPE purification system for EVs based stratification of BC, LC and HC management

Question 7

Work package WP7 – Prototype validation for GMP compliant manufacturing of EV based drug delivery system

Accepted Answer

Objectives
1)Validation of the PROTOTYPE purification system for isolation of PDEVs; 2) Development of efficient drug loading strategy to PDEVs;
3) Determination of the in vitro effect of drug-loaded PDEVs on the selected BC, HC and LC, by monitoring cell viability
4) Determination of the in vivo effect of drug-loaded PDEVs on orthotopic BC xenograft model.

Work packages